Genome-Wide Screen Reveals Valosin-Containing Protein Requirement for Coronavirus Exit from Endosomes.
Identifieur interne : 001782 ( Main/Exploration ); précédent : 001781; suivant : 001783Genome-Wide Screen Reveals Valosin-Containing Protein Requirement for Coronavirus Exit from Endosomes.
Auteurs : Hui Hui Wong [Singapour] ; Pankaj Kumar [Singapour] ; Felicia Pei Ling Tay [Singapour] ; Dimitri Moreau [Singapour] ; Ding Xiang Liu [Singapour] ; Frédéric Bard [Singapour]Source :
- Journal of virology [ 1098-5514 ] ; 2015.
Descripteurs français
- KwdFr :
- Adenosine triphosphatases (génétique), Adenosine triphosphatases (métabolisme), Animaux, Annotation de séquence moléculaire, Cellules Vero, Endosomes (virologie), Humains, Infections à coronavirus (transmission), Libération de particules virales (physiologie), Lignée cellulaire, Petit ARN interférent (génétique), Protéines du cycle cellulaire (génétique), Protéines du cycle cellulaire (métabolisme), Technique d'immunofluorescence, Technique de Western, Virus de la bronchite infectieuse (physiologie), Étude d'association pangénomique.
- MESH :
- génétique : Adenosine triphosphatases, Petit ARN interférent, Protéines du cycle cellulaire.
- métabolisme : Adenosine triphosphatases, Protéines du cycle cellulaire.
- physiologie : Libération de particules virales, Virus de la bronchite infectieuse.
- virologie : Endosomes.
- transmission : Animaux, Annotation de séquence moléculaire, Cellules Vero, Humains, Infections à coronavirus, Lignée cellulaire, Technique d'immunofluorescence, Technique de Western, Étude d'association pangénomique.
English descriptors
- KwdEn :
- Adenosine Triphosphatases (genetics), Adenosine Triphosphatases (metabolism), Animals, Blotting, Western, Cell Cycle Proteins (genetics), Cell Cycle Proteins (metabolism), Cell Line, Chlorocebus aethiops, Coronavirus Infections (transmission), Endosomes (virology), Fluorescent Antibody Technique, Genome-Wide Association Study, Humans, Infectious bronchitis virus (physiology), Molecular Sequence Annotation, RNA, Small Interfering (genetics), Valosin Containing Protein, Vero Cells, Virus Release (physiology).
- MESH :
- chemical , genetics : Adenosine Triphosphatases, Cell Cycle Proteins, RNA, Small Interfering.
- chemical , metabolism : Adenosine Triphosphatases, Cell Cycle Proteins.
- physiology : Infectious bronchitis virus, Virus Release.
- transmission : Coronavirus Infections.
- virology : Endosomes.
- Animals, Blotting, Western, Cell Line, Chlorocebus aethiops, Fluorescent Antibody Technique, Genome-Wide Association Study, Humans, Molecular Sequence Annotation, Valosin Containing Protein, Vero Cells.
Abstract
Coronaviruses are RNA viruses with a large zoonotic reservoir and propensity for host switching, representing a real threat for public health, as evidenced by severe acute respiratory syndrome (SARS) and the emerging Middle East respiratory syndrome (MERS). Cellular factors required for their replication are poorly understood. Using genome-wide small interfering RNA (siRNA) screening, we identified 83 novel genes supporting infectious bronchitis virus (IBV) replication in human cells. Thirty of these hits can be placed in a network of interactions with viral proteins and are involved in RNA splicing, membrane trafficking, and ubiquitin conjugation. In addition, our screen reveals an unexpected role for valosin-containing protein (VCP/p97) in early steps of infection. Loss of VCP inhibits a previously uncharacterized degradation of the nucleocapsid N protein. This inhibition derives from virus accumulation in early endosomes, suggesting a role for VCP in the maturation of virus-loaded endosomes. The several host factors identified in this study may provide avenues for targeted therapeutics.
DOI: 10.1128/JVI.01360-15
PubMed: 26311884
Affiliations:
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Coronavirus Exit from Endosomes.</title><author><name sortKey="Wong, Hui Hui" sort="Wong, Hui Hui" uniqKey="Wong H" first="Hui Hui" last="Wong">Hui Hui Wong</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cell Biology/A*STAR, Singapore, Singapore Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Institute of Molecular and Cell Biology/A*STAR, Singapore, Singapore Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Kumar, Pankaj" sort="Kumar, Pankaj" uniqKey="Kumar P" first="Pankaj" last="Kumar">Pankaj Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology/A*STAR, Singapore, 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Bard</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cell Biology/A*STAR, Singapore, Singapore Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore dxliu@ntu.edu.sg fbard@imcb.a-star.edu.sg.</nlm:affiliation><country wicri:rule="url">Singapour</country><wicri:regionArea>Institute of Molecular and Cell Biology/A*STAR, Singapore, Singapore Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphatases (genetics)</term><term>Adenosine 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(transmission)</term><term>Libération de particules virales (physiologie)</term><term>Lignée cellulaire</term><term>Petit ARN interférent (génétique)</term><term>Protéines du cycle cellulaire (génétique)</term><term>Protéines du cycle cellulaire (métabolisme)</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Virus de la bronchite infectieuse (physiologie)</term><term>Étude d'association pangénomique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Adenosine Triphosphatases</term><term>Cell Cycle Proteins</term><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenosine Triphosphatases</term><term>Cell Cycle Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adenosine triphosphatases</term><term>Petit ARN interférent</term><term>Protéines du cycle cellulaire</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adenosine triphosphatases</term><term>Protéines du cycle cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Libération de particules virales</term><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Infectious bronchitis virus</term><term>Virus Release</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Endosomes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Endosomes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Fluorescent Antibody Technique</term><term>Genome-Wide Association Study</term><term>Humans</term><term>Molecular Sequence Annotation</term><term>Valosin Containing Protein</term><term>Vero Cells</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="fr"><term>Animaux</term><term>Annotation de séquence moléculaire</term><term>Cellules Vero</term><term>Humains</term><term>Infections à coronavirus</term><term>Lignée cellulaire</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Étude d'association pangénomique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronaviruses are RNA viruses with a large zoonotic reservoir and propensity for host switching, representing a real threat for public health, as evidenced by severe acute respiratory syndrome (SARS) and the emerging Middle East respiratory syndrome (MERS). Cellular factors required for their replication are poorly understood. Using genome-wide small interfering RNA (siRNA) screening, we identified 83 novel genes supporting infectious bronchitis virus (IBV) replication in human cells. Thirty of these hits can be placed in a network of interactions with viral proteins and are involved in RNA splicing, membrane trafficking, and ubiquitin conjugation. In addition, our screen reveals an unexpected role for valosin-containing protein (VCP/p97) in early steps of infection. Loss of VCP inhibits a previously uncharacterized degradation of the nucleocapsid N protein. This inhibition derives from virus accumulation in early endosomes, suggesting a role for VCP in the maturation of virus-loaded endosomes. The several host factors identified in this study may provide avenues for targeted therapeutics.</div></front></TEI><affiliations><list><country><li>Singapour</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Wong, Hui Hui" sort="Wong, Hui Hui" uniqKey="Wong H" first="Hui Hui" last="Wong">Hui Hui Wong</name></noRegion><name sortKey="Bard, Frederic" sort="Bard, Frederic" uniqKey="Bard F" first="Frédéric" last="Bard">Frédéric Bard</name><name sortKey="Kumar, Pankaj" sort="Kumar, Pankaj" uniqKey="Kumar P" first="Pankaj" last="Kumar">Pankaj Kumar</name><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name><name sortKey="Moreau, Dimitri" sort="Moreau, Dimitri" uniqKey="Moreau D" first="Dimitri" last="Moreau">Dimitri Moreau</name><name sortKey="Tay, Felicia Pei Ling" sort="Tay, Felicia Pei Ling" uniqKey="Tay F" first="Felicia Pei Ling" last="Tay">Felicia Pei Ling Tay</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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